| Species |
Bovine |
| Protein Construction |
PDGF-BB (Ser82-Thr190)
Accession # B1H0W5 |
|
| Purity |
≥ 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.1 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 20.00 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the ED50, the calculated specific activity is approximately > 0.5 × 105 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay. |
| Expression System |
P. pastoris |
| Theoretical Molecular Weight |
12 kDa |
| Apparent Molecular Weight |
10-18 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in 20 mM NaAc, pH 5.0. |
| Reconstitution |
Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 °C. |
The ED50 as determined by a cell proliferation assay using mice Balb/c 3T3 cells is 9.86 ng/ml.
Lane 1: 2 µg of PDGF-BB, Bovine, reducing (R)
The purity is greater than 95% as analyzed by SDS-PAGE
| Target Background |
Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in an animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, and PDGF-AB. It is involved in many biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β). |
| Synonyms |
PDGFBB; PDGF-BB |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
