| Species |
Human |
| Protein Construction |
Her2, Avi & His, Human ( Thr23-Thr652)
Accession # P04626-1 |
His tag |
Avi tag |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Immobilized Her2, Avi & His, Human (Cat.No.: Z03806) at 0.5 μg/ml can bind Anti-Her2 Antibody. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
72.3 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Target Background |
ErbB2, also known as Neu and Her2, is a type I membrane glycoprotein belonging to the ErbB family of tyrosine kinase receptors. It serves as a receptor for the epidermal growth factor (EGF) family of growth factors. Upon activation, ERBB2 triggers the MEMO1-RHOA-DIAPH1 signaling pathway, leading to the phosphorylation and inhibition of GSK3B at the cell membrane. |
| Synonyms |
EGFR2; ErbB2; HER2; HER-2; TKR1; ERBB2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
