| Species |
Human |
| Protein Construction |
VEGFR2, Avi & His, Human (Ala20-Glu764 )
Accession # P35968-1 |
His tag |
Avi tag |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Immobilized VEGFR2, Avi & His, Human (Cat.No.: Z03811) at 0.5 μg/ml can bind AntiVEGFR2 Antibody. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
86.2 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Target Background |
The kinase insert domain receptor (KDR), also known as vascular endothelial growth factor receptor 2 (VEGFR-2), is a type IV receptor tyrosine kinase that plays a crucial role in various biological processes, including embryonic vasculature development, angiogenesis regulation, cell survival, migration, macrophage function, chemotaxis, and cancer cell invasion. It acts as a cell-surface receptor for VEGFA, VEGFB, and PGF. The human gene encoding KDR is also known as CD309 and Flk1 (Fetal Liver Kinase 1). VEGFR2 is a key regulator of angiogenesis. |
| Synonyms |
CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
