| Species |
Human |
| Protein Construction |
The Protein is composed of CD3E and CD3D
CD3E, His, Human (Asp23-Asp126)
Accession # P07766 |
His tag |
CD3D, His, Human (Phe22-Ala105)
Accession # P04234 |
His tag |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Immobilized CD3E&CD3D, His, Human (Cat.No.: Z03816) at 2 μg/ml can bind Anti-CD3 Antibody (OKT3). |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
17.9 kDa for CD3E
16.7 kDa for CD3D |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Target Background |
The T-cell surface glycoproteins CD3E and CD3D, also called CD3 epsilon and CD3 delta chain, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate the T-cell receptor (TCR), TCR signals are transmitted by the CD3 chains CD3D, CD3E, CD3G, and CD3Z. All CD3 chains have immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Synonyms |
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E & CD3D; CD3 delta & CD3 epsilon |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
