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Development of an ELISA-LC-MS hybrid assay for quantification of biotherapeutics.

Bioanalysis. 2018-09; 
ChenLinzhi，LiuSiyu，PagelsShirin，QuatranoCaitlin，RoosDave，PhilipElsy，ZhaoHenry，YuHon
Products/Services Used Details Operation
Protein Tools … Materials & methods. Reagent & materials. BI123ABC (∼39 kDa) was a proprietary biotherapeutic protein of Boehringer Ingelheim Pharmaceuticals, CT, USA and produced in-house. Peptides EGVSAIR and EGVSAI[ 13 C 6 , 15 N 4 -R] were produced by Genscript (NJ, USA) … Get A Quote

摘要

Magnetic bead immunocapture-LC-MS has been widely used for bioanalysis of biotherapeutic proteins. However， magnetic beads are difficult to be fully automated and more costly than ELISA plates.

关键词

ELISA，LC–MS，hybrid assay，immunocapture，magnetic beads，monkey，plasma，streptavidin p
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