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Quantification of Sulfotransferases 1A1 and 1A3/4 in Tissue Fractions and Cell Lines by Multiple Reaction Monitoring Mass Spectrometry

Drug Metab Lett. 2017-11;

YoshitakeSho, McKay-DailyMelissa, TanakaMasaki, Huang

Products/Services Used	Details	Operation
Peptide Synthesis	… Purities of >95% were determined using SDS-PAGE. Recombinant peptide standards for SULT1A1 and SULT1A3/4 were pur- chased from Genscript USA Inc. (Piscataway, NJ). Purities of >98% were determined using HPLC analysis …	Get A Quote

摘要

Within the sulfotransferase (SULT) superfamily of metabolic enzymes， SULT1A1 and 1A3/4 isoforms are of particular interest， due to their abilities to catalyze the sulfation of phenolic endobiotics and xenobiotics. Although the difference in their substrate specificity is well documented， an isoform-specific quantification method is still not available.