Melanotransferrin (MTf) is a protein associated with oncogenetic， developmental， and immune processes which function remains unclear. The MTf gene has been reported in numerous vertebrate and invertebrate species， including echinoderms. We now report the finding of four different MTfs in the transcriptome of the sea cucumber Holothuria glaberrima. Sequence studies and phylogenetic analyses were done to ascertain the similarities among the putative proteins and their relationship with other transferrin family members. The genes were shown to be differentially expressed in various holothurian organs and to respond differently when the animals were challenged with the immune system activator lipopolysaccharide (LPS). Moreover， the four genes were found to be highly overexpressed during the early stages of intestinal regeneration. The finding of four different genes in the holothurian is particularly surprising， because only one MTf gene has been reported in all other animal species sequenced to date. This finding， combined with the increase expression during intestinal regeneration， suggests a new possible function of MTf in organ regenerative processes.