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Distinct structural mechanisms determine substrate affinity and kinase activity of protein kinase Cα

ournal of Biological . 2018; 
Sangbae Lee‡, Titu Devamani§, Hyun Deok Song‡, Manbir Sandhu‡ , Adrien Larsen‡ , Ruth Sommese§ , Abhinandan Jain¶ , Nagarajan Vaidehi‡, and Sivaraj Sivaramakrishnan§
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Peptide Synthesis Reagents and peptides—Buffer chemicals were purchased from various commercial vendors and used without further purification. Peptides (1, 2, 4, 6, 9, 10, and 12) and the correspondingmutantpeptides(Figs.2and4)werecustom-synthesized by GenScript and solubilized in PKC buffer (20 mM HEPES(pH7.5),5mMMgCl2,0.5mMEGTA,and2mMDTT). Get A Quote

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