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C-terminal unfolding of an amyloidogenic β2-microglobulin fragment: ΔN6β2-microglobulin.

Amyloid. 2015-03;

MotomiyaYoshihiro，HigashimotoYuichiro，UjiYoshinori，SuenagaGenki，AndoY

Products/Services Used	Details	Operation
Proteins, Expression, Isolation and Analysis	… The inclusion bodies were dissolved with 8M urea, and one-step purification using Ni-Hi Trap column (GenScript, Piscataway, NJ) was performed The protein was finally solved in a storage buffer (consisting of 50 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 80) …	Get A Quote

摘要

A β2-microglobulin (β2m) fragment that lacks the first six amino acids， i.e.， ΔN6β2-microglobulin (ΔN6β2m)， is an endogenous， proteolytically derived， amyloidogenic fragment of β2m， the precursor protein in Aβ2M amyloidosis (dialysis-related amyloidosis). As reports suggest the importance of C-terminal unfolding for the amyloidogenicity of β2m， in this study we aimed to investigate conformational characteristics of ΔN6β2m related to amyloidogenicity. We also measured the concentration of an amyloidogenic intermediate of β2m with C-terminal unfolding (β2m92-99) in serum samples from 10 patients undergoing hemodialysis (HD).

关键词

Amyloidogenicity，capillary electrophoresis，dialysis-related amyloidosis，enzyme-linked immunosorbent assay，hemodialysis，monoclonal antibody 92-99，surface plasmon reson

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