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Cloning and characterization of a novel oocyte-specific gene encoding an F-Box protein in rainbow trout (Oncorhynchus mykiss).

Reprod. Biol. Endocrinol.. 2013; 
WangLei,TripuraniSwamy K,WannaWarapond,RexroadCaird E,YaoJi
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Proteins, Expression, Isolation and Analysis … peptide (CQKHSKRKVVSWGGT) of Fbxoo protein. The antibody was prepared and affinity purified commercially by GenScript Corporation (Piscataway, NJ). Western blot analysis. Frozen ovarian samples were homogenized … Get A Quote

摘要

Oocyte-specific genes play critical roles in oogenesis, folliculogenesis and early embryonic development. The objectives of this study were to characterize the expression of a novel oocyte-specific gene encoding an F-box protein during ovarian development in rainbow trout, and identify its potential interacting partners in rainbow trout oocytes.

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