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Nucleic Acid Purification & Analysis> | … Western blot. Cell lysates were prepared by lysing 425,000 of IMR-90 cells in NP-40 lysis buffer, boiling with sample buffer, and resolving on 4–12% Novex Bolt gradient gel, transferring to a PVDF membrane using Genscript's L1 eBlot and blotting for the specified antibodies … | Get A Quote |