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Quantitative Tetraplex Real-Time Polymerase Chain Reaction Assay with TaqMan Probes Discriminates Cattle， Buffalo， and Porcine Materials in Food Chain.

J Agric Food Chem. 2019-05;

HossainM A Motalib，AliMd Eaqub，SultanaSharmin，Asing，BonnySharmin Quazi，KaderMd Abdul，RahmanM Am

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Gene Synthesis	… These primers were described and published by previous reports ( Dai et al., 2015 ; Spychaj et al., 2016 ). The primers were synthesized by Nanjing GenScript Biotechnology Co., Ltd (Nanjing, China). Table 1 Primers for PCR amplification …	Get A Quote

摘要

Cattle， buffalo， and porcine materials are widely adulterated， and their quantification might safeguard health， religious， economic， and social sanctity. Recently， conventional polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) assays have been documented but they are just suitable for identification， cannot quantify adulterations. We described here a quantitative tetraplex real-time PCR assay with TaqMan Probes to quantify contributions from cattle， buffalo， and porcine materials simultaneously. Amplicon-sizes were very short (106-， 90-， and 146-bp for cattle， buffalo， and porcine) because longer targets could be broken down， bringing serious a... More