Products/Services Used | Details | Operation |
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Polyclonal Antibody Services> | Homozygous embryos for each allele were assayed for protein expression using a polyclonal antibody directed against the Ret ectodomain (residues 24-401; Genscript). | Get A Quote |
The RET receptor tyrosine kinase is crucial for the development of the enteric nervous system (ENS), acting as a receptor for Glial cell line-derived neurotrophic factor (GDNF) via GFR co-receptors. has a well-conserved RET homolog () that has been proposed to function independently of the co-receptor (). We find that is required for development of the stomatogastric (enteric) nervous system in both embryos and larvae, and its loss results in feeding defects. Live imaging analysis suggests that peristaltic waves are initiated but not propagated in mutant midguts. Examination of axons innervating the midgut reveals increased branching but the area covered by the branches is decreased. This phenotype can b... More