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Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase.

BMC Biotechnol.. 2019;

PradoGuilherme Souza，BamogoPingdwende Kader Aziz，de AbreuJoel Ant?nio Cordeiro，GilletFran?ois-Xavier，Dos SantosVanessa Olinto，SilvaMaria Cristina Mattar，BrizardJean-Paul，BemquererMarcelo Porto，BangratzMartine，BrugidouChristophe，SéréméDrissa，Grossi-de-SaMaria Fatima，LacombeSéve

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Catalog Antibody	The protein was probed by adding a primary specific anti-α-AIC3 rabbit IgG (GenScript) to the TBS-T buffer (1:2500 of antibody:buffer, or at 0.... The samples were discarded, washed and incubated together with 100 μL of a primary anti-α-AIC3 antibody (GenScript) diluted in PBS-T buffer with 1% gelatin (1:1000 of anti- body:buffer, v/v, or at 1 μg.	Get A Quote

摘要

Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance， molecular evolution strategies have been used on genes encoding these proteins to generate thousands of variants with new or improved functions. The cotton boll weevil (Anthonomus grandis) is one of the major pests of cotton in the Americas. An α-amylase inhibitor (α-AIC3) variant previously developed via molecular evolution strategy showed inhibitory activity against A. grandis α-amylase (AGA).