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| Codon Optimization | Garcia-Echauri and Cardineau Plant Methods (2015) 11:27 Page 6 of 7 TetX open reading frame [native sequence from En- terobacteriaceae bacterium; Genbank : JQ990987] was synthesized de novo at GenScript (Piscataway, NJ, USA) with codons optimized for Chlamydomonas reinhardtii cytoplasmic expression under the control of constitutive beta 2 tubulin promoter [31] and chlamyopsin1 3′UTR [11]; the plasmid was named Btetx (Figure 1). | Get A Quote |
Transformation of microalgae to obtain recombinant proteins, lipids or metabolites of economic value is of growing interest due to low costs associated with culture growth and scaling up. At present there are only three stable nuclear selection markers for the transformation of Chlamydomonas reinhardtii, which is the most commonly transformed microalgae, specifically: the aminoglycoside phosphotransferaseses aph7and aphVIII and the phleomycin resistance ble gene. As several microalgae are resistant to some of the antibiotics associated with the mentioned resistance genes, we have developed another alternative, tetX, a NADP-requiring Oxidoreductase that hydroxylates tetracycline substrates. We provide evidence t... More
