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| Gene Synthesis | DEGs possibly associated with cellulose synthesis including cellulose synthase A, NAC and MYB transcription factors were subjected to qRT-PCR detection. Gene-specific primers were designed using online primer design software (https://www.genscript.com/ssl-bin/app/primer) (Supplementary Table S1) and melting curve analysis was used to confirm specificity. Total RNAs from four stage rhizomes were isolated using the above-mentioned protocol and. cDNAs were generated using the RevertAid™ First Strand cDNA Synthesis Kit (Thermo Scientific, USA). QRT-PCR was performed using a Fast SYBR Mixture (CWBIO, Beijing, China) on a real-time thermal cycler (qTower2.2, Analytic Jena, Germany). The program was as follows: 95 °C incubation for 10 min, then 40 cycles of 95 °C for 15 s and 60 °C for 60 s. For all qPCR experiments, three biological replicates were performed. Relative expression levels were calculated based on the 2−ΔΔCt method using tubulin (Unigene17107_All) as a reference gene. | Get A Quote |
Ginger is a popular vegetable crop primarily consumed in Chongqing and Sichuan, China. The rhizome becomes tough and fibrous rapidly with the tissue maturation, resulting in loss of edible quality. To characterize biochemical and molecular mechanisms of rhizome texture modification, we investigated the changes of the cell wall components, hormones and transcriptome profiles during rhizome development. With rhizomes maturation, the contents of cellulose and hemicellulose increased gradually, and no obvious change was observed in lignin accumulation. The levels of ABA and cytokinin exhibited a gradual decline during maturation of ginger rhizome, which were negatively correlated with cellulose content, while GA3 ... More
