| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | For generation of the concentrated cells for counter-screening hybridomas, each culture (Table 1S) was centrifuged, resuspended in an equal volume of PBS, then 1 mL of each culture was pooled and heated at boiling temperature for 10 min to inactivate the cells. The pooled, heat-killed sample was sent to the contract provider (Genscript) for counter-screening the hybridoma cell cultures. All experiments utilized at least three individual biological replicates. | Get A Quote |
Rathayibacter toxicus is Gram-positive bacterium causing gummosis of forage grasses, and it produces a corynetoxin responsible for livestock poisonings in Australia. R. toxicus is a USDA APHIS-listed Select Agent, threatening seed and livestock production areas in the U.S. We therefore are developing diagnostic assays to prepare for the introduction of the pathogen into the U.S. Using proteomic data, we identified proteins expressed in R. toxicus but not in other Rathayibacter spp. Recombinant proteins corresponding to extracellular domains of candidate proteins were used to raise polyclonal and monoclonal antibodies. Antibodies were evaluated for sensitivity and specificity in immunoassays against a pan... More
