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Heterologous expression of a mutant Orange gene from Brassica oleracea increases carotenoids and induces phenotypic changes in the microalga Chlamydomonas …

Algal Research. 2020; 
Sujata Kumaria, Chaitali Viraa, Arvind M. Lalib, Gunjan Prakasha, ⁎
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Gene Synthesis Mutant Or genes from B. oleracea (mBoOr) and from C. reinhardtii (mCrOr) were synthesized at GenScript Inc. (USA). Get A Quote

摘要

In plants, a mutation in the Orange (Or) gene is known to enhance the accumulation of carotenoids in comparison to the wild-type (WT) allele. We investigated the effect of a point mutation in the Or gene of Brassica oleracea on its protein structure via in silico analysis. Subsequently we studied the effect of the Or mutation on carotenoid regulation in Chlamydomonas reinhardtii. The Or mutation in Brassica oleracea—mBoOr (R88H)—results in a small β-sheet in place of the α-helix that is present in the WT protein, and was found to be associated with enhanced carotenoid content when screened in Escherichia coli. The expression of the equivalent mutant from C. reinhardtii—mCrOr (R106H)—did no... More

关键词

Carotenoids, Orange, Palmelloids, Multicellularity, Chlamydomonas reinhardtii
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