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Development of a monoclonal antibody based-ELISA for the detection of chloramphenicol in shrimp, feed and milk samples and validation by LC-MS/MS coupled with …

Analytical Methods. 2019-01; 
Chang Liu,a Diandian Deng,a Di Xu,a Kang Wu,*b Hong Yang,c Kang Zhao,a Jianguo Li ORCID logo *a and Anping Deng ORCID logo *a
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Proteins, Expression, Isolation and Analysis … The CNBr-activated Sepharose-4B gel was bought from Pharmacia Biotech (Uppsala, Sweden) Protein G resin was purchased from GenScript (Nanjing, China) The myeloma cell line of SP2/0 was from the Medical College of Soochow University (Soochow, China) … Get A Quote

摘要

A simple and fast ultra-high-performance liquid chromatography method with UV detection for the separation and quantification of omeprazole and the impurities of omeprazole and methylparaben (the internal standard) in six extemporaneous suspensions has been developed and fully validated. Isocratic elution was carried out using a Kinetex™ C18 column, 50 × 2.1 mm packed with 1.7 μm porous shell particles, and the mobile phase comprised a mixture of phosphate buffer and acetonitrile. The separation of all compounds was achieved within 2 min. This method was successfully applied for stability evaluation of the developed formulations, which are now being used in the therapy of acid-related disorders in paediatri... More

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