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Protease activation of split green fluorescent protein.

Chembiochem.. 2010-11;  11(16):2259-63
Brian P. Callahan, Matthew J. Stanger, Marlene Belfort. Wadsworth Center, NYS Department of Health, P. O. Box 22002, Albany, NY 12201-2002 (USA)
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摘要

Cut and glow: Site-specific proteolysis triggers the assembly of green fluorescent protein fragments, providing a novel reporter of protease activity in vitro and in live E. coli.

关键词

enzyme catalysis;fluorescent probes;peptides;protein engineering
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