Re: Microfluidic Sorting Selects Sperm for Clinical Use with Reduced DNA Damage Compared to Density Gradient Centrifugation with Swim-up in Split Semen Samples
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Proteins, Expression, Isolation and Analysis> |
… Quantitative polymerase chain reaction (qPCR) was performed combining standards or 1μl
cDNA with PCR buffer containing SYBR Green I (Sigma Chemical Co, St. Louis, Missouri),
Taq polymerase (Genscript, Piscataway, NJ), and primers for IGF1R … |
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