Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding
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, DNA encoding the proteins was codon optimized (Genscript) and cloned into pET11a using NdeI/BamHI PfEMP11–60 K4A (PFL1960w), was codon optimized (Genscript) and cloned into pGBP1303CmycGFP6His by NdeI synthesized by Epoch Biolabs or Genscript, and cloned in-frame with GFP in pGlux |
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