GenScript USA Inc., and inserted into pColdIV vector having an ampicillin resistant gene (Takara Bio Inc.,) as shown in Fig. ...">

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The use of the condensed single protein production system for isotope-labeled outer membrane proteins, OmpA and OmpX in E. coli.

Mol Biotechnol.. 2011-03;  47(3):205-210
Vaiphei ST, Tang Y, Montelione GT, Inouye M. Department of Biochemistry, Robert Wood Johnson Medical School and Centre for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA.
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摘要

The flagellum-specific export system is a specialized type III export machinery, which exports external flagellar proteins through the central channel of the flagellar filament. A number of evidence indicates that short segments within the disordered N-terminal region of flagellar axial proteins are recognized by the flagellum-specific export apparatus. Recently, we have demonstrated that the 26-47 segment of Salmonella typhimurium flagellin is capable of mediating flagellar export. N-terminal flagellin segments containing the export signal combined with a hexahistidine tag can be attached to heterologous proteins (preferentially in the size range of 9-40 kDa) facilitating their secreted expression and easy pur... More

关键词

NMR; OmpA; OmpX; MazF
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