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Prevalent and immunodominant CD8 T cell epitopes are conserved in SARS-CoV-2 variants

Cell Rep .. 2023-01; 
Saskia Meyer , Isaac Blaas , Ravi Chand Bollineni , Marina Delic-Sarac , Trung T Tran , Cathrine Knetter , Ke-Zheng Dai , Torfinn Støve Madssen , John T Vaage , Alice Gustavsen , Weiwen Yang , Lise Sofie Haug Nissen-Meyer , Karolos Douvlataniotis , Maarja Laos , Morten Milek Nielsen , Bernd Thiede , Arne Søraas , Fridtjof Lund-Johansen , Even H Rustad, Johanna Olweus
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Peptide Synthesis Custom made synthetic peptides GenScript/ In total, 123 peptides derived from spike,nucleocapsid, envelope, membrane, and ORF3a were successfully synthesized at Genscript (purity >70%) and dissolved in DMSO. Get A Quote

摘要

The emergence of SARS-CoV-2 variants of concern (VOC) is driven by mutations that mediate escape from neutralizing antibodies. There is also evidence that mutations can cause loss of T cell epitopes. However, studies on viral escape from T cell immunity have been hampered by uncertain estimates of epitope prevalence. Here, we map and quantify CD8 T cell responses to SARS-CoV-2-specific minimal epitopes in blood drawn from April to June 2020 from 83 COVID-19 convalescents. Among 37 HLA ligands eluted from five prevalent alleles and an additional 86 predicted binders, we identify 29 epitopes with an immunoprevalence ranging from 3% to 100% among individuals expressing the relevant HLA allele. Mutations in VOC are... More

关键词

CD8 T cell; CP: Immunology; CP: Microbiology; SARS-CoV-2; epitope; immunodominance; immunoprevalence.
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