| Specificity |
This Antibody recognizes both S1 tagged fusion proteins. |
| Host Species |
Rabbit |
| Immunogen |
S1 epitope tag peptide NANNPDWDF conjugated to KLH |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
| Western Blot |
1:100-1:1,000 |
|
| Form |
Liquid |
| Storage Buffer |
1×PBS, pH7.4, containing 0.02% sodium azide |
| Concentration |
1 mg/ml |
| Storage Instructions |
The product remains stable for up to 1 year at -20 °C from the date of receipt. The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Western blot analysis of S1-tagged fusion protein using S1-tag Antibody, pAb, Rabbit (GenScript, A00679, 2 µg/ml)
The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 48 kD
Observed Size: 48 kD
Western blot analysis of S1 tagged fusion proteins expressed in E. coli cell lysate using S1-tag Antibody (GenScript, A00679, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
| Target Background |
S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region.Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems.The Rabbit Anti-S1-tag polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. |
| Synonyms |
Rabbit Anti-S1 pAb;Anti-S1-tag pAb |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
