| Specificity |
The product is specific for Humanized VHH antibodies and Camelid (Llama, Alpaca, and Camel) heavy chain antibodies (IgG2&3), but does not recognize the camelid conventional antibody (IgG1). |
| Host Species |
Rabbit |
| Immunogen |
VHH antibody |
| Species Reactivity |
It has no cross-reactivity with mouse, rat, rabbit, or goat immunoglobulins. |
| Conjugate |
Biotin |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
1:5,000-1:10,000 |
| Flow Cytometry |
1:100-1:1,000 |
|
| Form |
Liquid |
| Storage Buffer |
Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.02% sodium azide and 50% glycerol. |
| Concentration |
0.5 mg/ml |
| Storage Instructions |
Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification |
Protein A affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
45H8 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
![MonoRab™ Rabbit Anti-Humanized VHH Antibody [Biotin], MAb](/product/documents/down?doc_name=A02166_FACS.png&file=scm_files/productFile_notes/2022/09/01/27caf993-46df-47da-9ebc-85066cae3cbe.png "MonoRab™ Rabbit Anti-Humanized VHH Antibody [Biotin], MAb")
FACS binding of a series of dilutions of MonoRab™ Rabbit Anti-Humanized VHH Antibody [Biotin], mAb (GenScript, A02166) with Jurkat cells and samples containing 20% of VHH-based Jurkat-CAR cells. The proportion of VHH-based Jurkat-CAR detected by A02166 was consistent with the theoretical value, indicating that A0166 shows excellent binding activity on VHH-based Jurkat-CAR cells.
| Target Background |
A camelid single-domain antibody (sdAb, called Nanobody by Ablynx, the developer) is a peptide chain of about 110 amino acids long, comprising one variable domain of a heavy-chain antibody, these are called VHH fragments. Like a whole antibody, it is able to bind selectively to a specific antigen. Single-domain antibodies allow a broad range of applications in biotechnical as well as therapeutic uses due to their small size, simple production, and high affinity. However, their sequences may increase the risk of immunogenicity and anti-drug antibody (ADA) development in humans, and thus, sdAbs are routinely humanized during development. |
| Synonyms |
Biotin conjugated rabbit anti-Humanized VHH, single-domain antibody monoclonal antibody (Min X Ms, Rt, Rb, Gt). |
| Reference |
1. Immunogenicity and humanization of single-domain antibodies.
2. Properties, production, and applications of camelid single-domain antibody fragments.
|
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
