| Specificity |
The product is specific for human CD28. |
| Host Species |
Mouse |
| Immunogen |
Recombinant human CD28 |
| Species Reactivity |
Human |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The suitability of this antibody for applications other than those listed below has not been determined. For flow cytometric staining, the following concentration range is recommended starting points for this product and is suggested that the reagent be titrated for optimal performance for each application. |
| Application |
Recommended Usage |
| Flow Cytometry |
≤ 2 μg per million cells in 100 μl volume. It is strongly recommended that the reagent should be titrated for optimal performance for each application. |
|
| Form |
Liquid |
| Storage Buffer |
Supplied in 10 mM Sodium Citrate, 150 mM NaCl, pH 6.5. |
| Concentration |
1 mg/ml |
| Endotoxin Level |
< 0.5 EU/μg by gel clotting method |
| Storage Instructions |
The product remains stable up to 12 months at 4 °C from date of receipt. For longer time storage, store at -80 °C. Avoid repeated freeze-thaw cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG1κ |
| Clonality |
Monoclonal |
| Note |
GenScript can customize this product per customer's request including conjugations, product size, buffer components, etc. |
Flow cytometric analysis of CD28 expression on human peripheral blood lymphocytes. Human PBMC stained with Anti-Human CD28 Antibody (F105), mAb, Mouse (GenScript, Cat. No. A02213. Red) or with mouse IgG1 isotype control (Gray), followed by goat anti-mouse IgG PE-conjugated antibody. Data shown was gated on CD45 (+)/SSC Low lymphocytes.
| Target Background |
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. It plays an important role in cell proliferation and cytokine production and promotion of T-cell survival. It also enhances the production of IL-4 and IL-10 in T cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. |
| Synonyms |
CD28; TP44; T-cell-specific surface glycoprotein CD28; Cluster of differentiation 28 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
