BLI (Biolayer interferometry) binding affinity measurements of MonoRab™ Anti-Risankizumab Antibody (32A12), mAb, Rabbit (GenScript, A02322) to Risankizumab.
Risankizumab captured on HFC (Anti-Human IgG Fc) Probes can bind MonoRab™ Anti-Risankizumab Antibody (32A12), mAb, Rabbit with a dissociation constant (KD) of less than 1 pM.

Anti-Risankizumab Antibody (32A12) inhibits the binding of Risankizumab to IL-23.

Human IL23A Protein was coated at a concentration of 1 μg/mL, followed by the addition of Risankizumab at 0.6 μg/mL and Anti-Risankizumab antibody that was serially diluted starting from 10 μg/mL. Detection was performed using Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) diluted at 1:10,000.
MonoRab™ Anti-Risankizumab Antibody (32A12), mAb, Rabbit (GenScript, A02322) demonstrated inhibitory properties with an IC₅₀ value of 0.18 µg/mL.

Standard Curve of Risankizumab Sandwich ELISA by A02322 (32A12) and anti-human IgG Fc antibody.
In this Sandwich ELISA assay, MonoRab™ Anti-Risankizumab Antibody (32A12), mAb, Rabbit (GenScript, A02322) was coated at a concentration of 1 μg/ml, and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) was used as a detection antibody at a dilution of 1:10,000.
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99999. The typicaldynamic range of the assay is 0.14 - 11 ng/mL.

Standard Curve of Risankizumab Sandwich ELISA by A02321 (3A11) and A02322 (32A12).
In this Sandwich ELISA assay, MonoRab™ Anti-Risankizumab Antibody (3A11), mAb, Rabbit (GenScript, A02321) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Risankizumab Antibody (32A12), mAb, Rabbit (GenScript, A02322) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL. GenScript can provide customized conjugation service for this product per customer's request.
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99957. The typicaldynamic range of the assay is 0.078 - 2.5 ng/mL.

MRD Analysis of the Detection of Risankizumab in Biological Matrix.
The MRD is the minimum dilution necessary for the detection of Risankizumab in biological matrix with least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggested that MRD was as 1:10.
In this ELISA assay, MonoRab™ Anti-Risankizumab Antibody (3A11), mAb, Rabbit (GenScript, A02321) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Risankizumab Antibody (32A12), mAb, Rabbit (GenScript, A02322) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL.

Specificity analysis of the detection of Risankizumab.

Risankizumab standard samples at 6 concentrations were spiked with 10 µg/mL of human IgG1. The test result demonstrated that the high concentration of human IgG1 did not interfere with the detection of Risankizumab.
In this ELISA assay, MonoRab™ Anti-Risankizumab Antibody (3A11), mAb, Rabbit (GenScript, A02321) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Risankizumab Antibody (32A12), mAb, Rabbit (GenScript, A02322) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL.