| Product Description |
GenScript BSA ELISA Kit, 2G (Cat. No.
L00976) is a Sandwich ELISA Kit that
is designed for quantitative measurement of bovine serum albumin (BSA) in
testing samples. These outstanding features make it a great solution for
routine BSA impurity detection, quality control and lot release, as well as
purification process optimization.
Most
commercial serum-free media are formulated with various biological macromolecules
that can replace the function of serum, such as bovine serum albumin (BSA),
transferrin, and insulin. The use of serum-free media can greatly reduce the
amount of potential impurities, but the final product serving therapeutic
purpose should be highly purified and quality controlled. Hence, it is
necessary to test for trace media impurities, and the residual concentration of
BSA is usually an important indicator for quality control
in production. |
| Linearity Range |
0.5 ng/mL - 35 ng/mL |
| Sensitivity |
LoB: 0.14 ng/mL
LoQ: 0.5 ng/mL |
| Kit Contents |
| Component |
Quantity/Size |
Part No. |
| Capture Plate |
1 plate |
J1-80 |
| Standard Stock |
1 vial (50 μL) |
J1-10 |
| Biotin Anti-BSA Antibody |
1 bottle (12 mL) |
J1-20 |
| Streptavidin-HRP |
1 bottle (12 mL) |
E1-30 |
| Sample Dilution Buffer |
1 bottle (40 mL) |
J1-60 |
| TMB Solution |
1 bottle (12 mL) |
A1-40 |
| 20× Wash Solution (2% Tween-20) |
1 bottle (40 mL) |
F1-70 |
| Stop Solution |
1 bottle (6 mL) |
A1-50 |
| Plate Sealer |
2 pieces |
N/A |
|
| Storage |
The unopened kit is
stable for at least 12 months from the date of manufacture at 2°C to 8°C, and
the opened kit is stable for up to 1 month from the date of opening at 2°C to
8°C. |
| Assay Principle |
BSA ELISA Kit, 2G utilizes two anti-BSA monoclonal antibodies which bind to different epitopes of BSA. When the sample and the anti-BSA detection antibody conjugated with biotin are added to the plate (Capture Plate) coated with the anti-BSA capture antibody, the capture antibody and the detection antibody (Biotin Anti-BSA Antibody) bind to the BSA in the sample in a sandwich format. Streptavidin-Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the Biotin Anti-BSA Antibody. After washing steps, 3,3',5,5'-Tetramethylbenzidine solution (TMB Solution) is added, resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color can be read at 450 nm and 650 nm by a microplate reader. The quantity of BSA in the sample is precisely quantified against a BSA standard curve.
Schematic diagram of BSA ELISA Kit, 2G |
| Reference |
1, Francis G L. Albumin and mammalian cell culture:
implications for biotechnology applications [J]. Cytotechnology, 2010, 62(1): 1-16.
2, CLSI document EP06-A (Evaluation of the Linearity
of Quantitative Measurement Procedures: A Statistical Approach; Approved
Guideline, 2nd Edition).
3, CLSI document EP17-A2 (Evaluation of Detection Capability for Clinical
Laboratory Measurement Procedures; Approved Guideline—Second Edition). |
BSA ELISA Kit, 2G standard curve.
For
research use only. Not intended for human and animal therapeutic or diagnostic
use.
