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目录产品 » 蛋白检测和免疫学分析 » 蛋白分析试剂 » HRP-Protein G

HRP-Protein G

Protein G, a cell surface protein of Group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A. It contains 3 IgG binding domains and is engineered to delete albumin-binding region. HRP has been covalently coupled to Protein G to produce the HRP-Protein G, which can be used in detection systems such as immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry.
M00090
￥750

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产品属性
Reconstitution Reconstitute the lyophilized power with 1.0 ml of deionized water to a final HRP-Protein G concentration of 1 mg/ml.
Concentration 1 mg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal.
Storage The product remains stable in lyophilized form for at least 1 year if stored at 2-8°C, -20°C, or lower from the date of receipt. For longer storage, -20°C or below is recommended.
The reconstituted product can be stored for 2-3 weeks at 2-8°C or for up to 1 year at -20°C or below. Avoid repeated freezing and thawing cycles.

应用指导
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including primary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Dot blot: 1:2,000-1:10,000
Western blot: 1:2,000-1:10,000
ELISA: 1:10,000-1:50,000
Immunohistology: 1:1,000-1:10,000

技术背景
Target Background Protein G, a cell surface protein of Group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A. It contains 3 IgG binding domains and is engineered to delete albumin-binding region. HRP has been covalently coupled to Protein G to produce the HRP-Protein G, which can be used in detection systems such as immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry.

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