目录产品 » 稳定细胞系 » Human Recombinant CCKB Cholecystokinin Receptor Stable Cell Line

Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells.
The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to stimulation with CCKB agonist, CCK8. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of CCK8 (mean ± SD, n = 3). The EC₅₀ of CCK8 on CHO-K1/CCKB cells was 0.945 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

CHO-K1/CCKB Stable Cell Line

Human Recombinant CHO-K1/CCKB Stable Cell Line stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring the In Vitro function of CCKB.

产品编号	M00154
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description	Human Recombinant CHO-K1/CCKB Stable Cell Line stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring the In Vitro function of CCKB.
Applications	Functional assay for CCKB receptor (Calcium Flux)

产品简介

Expressed Gene	Codon optimized from NM_176875; no expressed tags
Target Protein	NP_795344.1
Host Cell	CHO-K1
Size	Two vials of frozen cells (1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability	Stable through more than 16 passages without significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Gibco), 45% FBS (Cat. No. A5669701, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)

技术背景

Gene Synonyms

CCK-2R; CCK-B; CCK2R; GASR; CCKBR

Background

Cholecystokinins (CCK) are derived from preprocholecystokinin. They are widely expressed in gastrointestinal tissues and in the CNS. There are two cholecystokinin receptors, referred to as A and B (CCKAR and CCKBR). CCKBR can be activated by the smaller CCK-4. In the CNS, CCKBR has been implicated in anxiety, depression, schizophrenia, depression, and opioid analgesia. In the stomach, CCK-2 mediates gastrin-stimulated gastric acid secretion.

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