Figure 1: Neurokinin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK2 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK2 receptor agonist, Neurokinin A. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neurokinin A (Mean ± SD, n = 2). The EC₅₀ of Neurokinin A on this cells was 46.5 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/NK2 Stable Cell Line

Tachykinins are peptides sharing a common C-terminal amino acid sequence: Phe-X-Gly-Leu-Met-NH2. This neuropeptide family is composed of substance P, neurokinin A, and neurokinin B, which are widely distributed in mammalian central and peripheral nervous systems. These three molecules serve as both neurotransmitters and neuromodulators. Their actions are mediated by binding with three distinct receptors, namely NK1, NK2, and NK3. In particular, NK2 is expressed in gastrointestinal tract. Activation of NK2 is chiefly responsible for the regulation of intestinal motor functions (both excitatory and inhibitory), secretions, inflammation, and visceral sensitivity. Antagonists of NK2 may be useful in the treatment of irritable bowel syndrome.

产品编号	M00200
规格	2 vials 大单询价
数量
价格	询价


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Synonyms

Human Recombinant NK2 Tachykinin Receptor Stable Cell Line

产品概述

Applications

Functional assays for NK2 receptor

产品简介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培养条件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

图例

Figure 1: Neurokinin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK2 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK2 receptor agonist, Neurokinin A. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neurokinin A (Mean ± SD, n = 2). The EC₅₀ of Neurokinin A on this cells was 46.5 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

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CHO-K1/NK2 Stable Cell Line

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