目录产品 » 稳定细胞系 » Human Recombinant Corticotropin Releasing Factor Receptor CRF2 Stable Cell Line

Figure 1: Dose dependent stimulation of intracellular cAMP accumulation upon treatment with Urocortin in CHO-K1/CRF2/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. # 62AM4PEC, Cisbio) and intracellular cAMP in CHO-K1/CRF2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by PHERAstar (BMG). The EC₅₀ of Urocortin on CHO-K1/CRF2/Gα15 cells was 0.0865 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is Ratio (665/620) and starts at Bottom and goes to Top along a sigmoid curve.

CHO-K1/CRF2/Gα15 Stable Cell Line

Recombinant CHO-K1/CRF2/Gα15 (cGMP) cells stably overexpress human corticotropin releasing factor receptor (CRF2) on the surface and contain high levels of G protein (Gs) to couple with the receptor in downstream signaling pathways.

产品编号	M00208
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description	Recombinant CHO-K1/CRF2/Gα15 (cGMP) cells stably overexpress human corticotropin releasing factor receptor (CRF2) on the surface and contain high levels of G protein (Gs) to couple with the receptor in downstream signaling pathways.
Applications	Functional assay for CRF2 receptor (cAMP accumulation assay)

产品简介

Expressed Gene	Codon optimized from NM_001883.5; no expressed tags
Target Protein	NP_001874.2
Host Cell	CHO-K1
Size	Two vials of frozen cells (1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. # LT07-318, Lonza).
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	45% culture medium, 45% FBS (Cat. # A5669701, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Complete Growth Medium	Ham’s F-12 K (Kaighn’s) (Cat. #21127, Life Technologies), 10% FBS
Culture Medium	Ham’s F-12 K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 200 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

技术背景

Gene Synonyms

CRF-RB; CRHR2; CRFR2; HM-CRF

Background

The corticotropin-releasing factor receptor 2 CRF2 is Gs-coupled GPCRs expressed in the brain, blood vessels and intestine that bind to corticotropin-releasing factor (CRF). The CRF is a 41-amino acid peptide that plays an important role in the integration of autonomic, neuroendocrine, and behavioral responses to stress. GenScript generated a clonal human CRF2-expressing cell line co-expressing Gα15 in the CHO-K1 host cells.

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