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目录产品 » 稳定细胞系 » Human Recombinant Melatonin MT1 Receptor Stable Cell Line
CHO-K1/MT1/Gα15 Stable Cell Line

Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist melatonin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of melatonin (Mean ± SD, n = 2). The EC50 of Melatonin on this cell was 2.3 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/MT1/Gα15 Stable Cell Line

Melatonin binds to two specific G-protein coupled receptors (GPCR), MT1 (MTNR1A/MEL1A) and MT2 (MTNR1B/MEL1B). MT1 receptors signal via inhibitory G proteins (Gαi and Gαo) leading to adenylate cyclase inhibition and possibly inositol phosphate stimulation in recombinant systems. In certain native tissues (e.g. sheep pars tuberalis, rat cerebral and caudal arteries) melatonin responses are presumably mediated through activation of MT1 receptors. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.
M00424
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Synonyms

MT1 receptor, ML1A, MEL1A, ML1A, Mel1a, MEL-1A-R, mel1a melatonin receptor, mel1a receptor, melatonin receptor 1A, melatonin receptor type 1A, MelR

产品概述
Applications Functional assay for MT1 receptor

产品简介
Storage Liquid nitrogen immediately upon delivery
Species Human

培养条件
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

图例
  • CHO-K1/MT1/Gα15 Stable Cell Line
    • CHO-K1/MT1/Gα15 Stable Cell Line

    Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist melatonin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of melatonin (Mean ± SD, n = 2). The EC50 of Melatonin on this cell was 2.3 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom


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