Figure 1. Trypsin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PAR2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist trypsin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of trypsin (Mean ± SD, n = 2). The EC₅₀ of Trypsin on this cell was 1.7 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/PAR2/Gα15 Stable Cell Line

Proteinase-activated receptors (PAR) are a subfamily of G-protein coupled, seven-transmembrane domain receptors, which are cleaved within the aminoterminal exodomain by certain serine proteinases at a specific peptide bond. Trypsin and mast cell tryptase, and more recently, the activated coagulation factors VIIa and Xa, have been identified as serine proteinases able to activate mammalian PAR-2. As already indicated, PAR-2 is believed to be involved in inflammation. This role for PAR-2 implies that elastase and cathepsin G would paradoxically display an anti-inflammatory property by disarming PAR-2.

产品编号	M00446
规格	2 vials 大单询价
数量
价格	询价


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Synonyms

Human Recombinant PAR2 Proteinase-activated Receptor Stable Cell Line

产品概述

Applications

Functional assay for PAR2 receptor

产品简介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培养条件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 µg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 µg/ml Hygromycin B (Cat. #10687010, Invitrogen)

图例

Figure 1. Trypsin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PAR2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist trypsin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of trypsin (Mean ± SD, n = 2). The EC₅₀ of Trypsin on this cell was 1.7 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

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CHO-K1/PAR2/Gα15 Stable Cell Line

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