目录产品 » Human Recombinant Glucagon-like Peptide 1 Receptor Stable Cell Line

Figure 1: GLP-1(7-37)-induced concentration-dependent stimulation of intracellular cAMP (Luciferase reporter) in HEK293/CRE-luc/GLP1R cells. After stimulation with GLP1R receptor agonist GLP-1(7-37), the cells are incubated with Fire-Lumi™ Luciferase Assay System (L00877C, Genscript) and the relative luminescence units (RLU) were recorded on PheraStar. The RLU were plotted against the log of the cumulative doses of GLP-1(7-37) (Mean ± SEM, n = 3). The EC50 of GLP-1(7-37) on this cell was 0.798 nM

HEK293/CRE-Luc/GLP1R Stable Cell Line

Recombinant HEK293 cells stably overexpress human glucagon-like peptide-1(GLP1R) on the surface and contain high levels of G protein (Gs) to couple with the receptor in downstream signaling pathways.

产品编号	M00562
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description	Recombinant HEK293 cells stably overexpress human glucagon-like peptide-1(GLP1R) on the surface and contain high levels of G protein (Gs) to couple with the receptor in downstream signaling pathways.
Applications	cAMP assay and Luciferase assay for GLP1R receptor.

产品简介

Expressed Gene	Codon Optimized from NM_002062; no expressed tags
Target Protein	NP_002053
Host Cell	HEK293
Quantity	2 vials of frozen cells (>1X10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. #LT07-318, Lonza).
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	90% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Complete Growth Medium	DMEM (Cat. #10566-016, Gibco), 10% FBS
Culture Medium	DMEM, 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

技术背景

Gene Synonyms

GLP-1

Background

GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning.

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