目录产品 » 稳定细胞系 » Human Recombinant CD32C 13Gln Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD32C 13Gln on CHO-K1 cells. The CHO-K1/CD32C 13Gln cells (blue) and the negative control CHO-K1 cells (red) were stained with APC Mouse Anti-Human CD32 (Cat. No. 559769, BD Biosciences).

CHO-K1/CD32C 13Gln Stable Cell Line

Recombinant CHO-K1 cells stably overexpress Homo sapiens cell-type natural killer cells Fc gamma receptor IIc1 (FcγRIIc / CD32C) on the cell surface. The surface expression of FcγRIIc is validated by FACS analysis. This stable cell line is recommended for evaluating the binding affinity between FcγRIIc and Fc region of antibodies, Fc fusion proteins, or antibody-based biologics.

产品编号	M00601
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description	Recombinant CHO-K1 cells stably overexpress Homo sapiens cell-type natural killer cells Fc gamma receptor IIc1 (FcγRIIc / CD32C) on the cell surface. The surface expression of FcγRIIc is validated by FACS analysis. This stable cell line is recommended for evaluating the binding affinity between FcγRIIc and Fc region of antibodies, Fc fusion proteins, or antibody-based biologics.
Applications	Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon optimized from U90938.1; no expressed tags
Target Protein	AAC12807.1
Host Cell	CHO-K1
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	95% Complete Growth Medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

技术背景

Gene Synonyms

CD32; FCG2; CD32C; CDW32; IGFR2; FCRIIC

Background

Human studies suggest expression of CD32C could help predict patient responses to vaccines. CD32C normally contains a termination codon, but 7%–15% of individuals carry a mutation that changes it into a full-length open reading frame. Individuals with this variant expressed CD32C on B cells and were more likely to respond to an anthrax vaccine than patients that did not express the variant. Individuals with the full-length CD32C variant also were more likely to develop the autoimmune disease systemic lupus erythematosus (SLE) than those without the variant.

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