目录产品 » 稳定细胞系 » Human Recombinant FcRn Stable Cell Line

Figure 1. FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells.
The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were probed using FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz Biotechnology) and PE conjugated Goat anti-mouse IgG (minimal x-reactivity) Antibody (Cat. No. 405307, Biolegend).

CHO-K1/FcRn Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human FcRn and B2M subunits. The B2M subunits enable stable expression of FcRn in cells. Association of FcRn alpha-chain with beta (2) m is also important for IgG binding. The surface expression of FcRn is validated by FACS analysis. This cell can be used in binding assay or as an immunogen.

产品编号	M00603
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description	Recombinant CHO-K1 cells stably overexpress human FcRn and B2M subunits. The B2M subunits enable stable expression of FcRn in cells. Association of FcRn alpha-chain with beta (2) m is also important for IgG binding. The surface expression of FcRn is validated by FACS analysis. This cell can be used in binding assay or as an immunogen.
Applications	Binding assay or use as immunogen

产品简介

Expressed Gene	Codon optimized from NM_004107 (for FcRn); AF072097 (for B2M); no expressed tags
Target Protein	NP_004098.1 (for FcRn), AAD48083.1 (for B2M)
Host Cell	CHO-K1
Size	Two vials of frozen cells (1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability	Stable through more than 15 passages without significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. A5669701, Gibco)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Gibco), 200 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

技术背景

Gene Synonyms	FCGRT, alpha-chain, FcgammaRn (for FcRn), and Beta-2-Microglobulin (for B2M)
Background	This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation.