目录产品 » Human Recombinant SARS-CoV-2 Spike Stable Cell Line

Figure 1: FACS analysis of cell surface expression of SARS-CoV-2 spike protein on CHO-K1 cells. CHO-K1/Spike cells (red) and negative control CHO-K1 cells (grey) were probed using ACE-2 Fc Chimera, Human (Cat. No. Z03516, GenScript) and Alexa Fluor 647 conjugated Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Cat. No. A-21445, Thermo Fisher).

CHO-K1/Spike Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human SARS-CoV-2 spike protein on their surface. The surface expression of SARS-CoV-2 spike protein is validated by FACS analysis. This stable cell line product is designed for screening antibodies against the SARS-CoV-2 spike protein, as well as measuring binding affinity and stability of antibody-based biologics that bind with spike protein. GenScript also offers spike protein expressing HEK293T stable cell line (Cat. No. M00804) for SARS-CoV-2 study.

产品编号	M00803
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description

Applications

Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon optimized from NC_045512.2; no expressed tags
Target Protein	YP_009724390.1
Host Cell	CHO-K1
Size	2 vials of frozen cells (>1X10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	Ham's F-12K (Kaighn's) Medium, 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/mL Puromycin (Cat. No. A11138-03, Gibco)

技术背景

Gene Synonyms

S; spike glycoprotein

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell, the infecting RNA is used to encode structural proteins that make virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control, and accessory proteins whose function has not been determined. The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronaviruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

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