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dsDNA_TRAC_EGFP

dsDNA_TRAC_EGFP
KIOK7
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Description dsDNA_TRAC_EGFP HDR donor template is high purity GenWandTM closed-end linear dsDNA designed for inserting EGFP into targeted TRAC locus. The resulting EGFP expression will be placed under the endogenous TCR promoter. 

Advantages of GenWandTM dsDNA donor template:
✔ Covalently closed ends protection for better accuracy
✔ More suitable for scale up
✔ High purity and sequence verified
✔ Research to GMP grade
Learn More>>> https://www.genscript.com/genwand-double-stranded-dna-synthesis-service.html

This dsDNA HDR donor template works in combination with SafeEdit TRAC sgRNA and Cas9 protein.
Sequence
GGTTTCCTTGAGTGGCAGGCCAGGCCTGGCCGTGAACGTTCACTGAAATCATGGCCTCTT
GGCCAAGATTGATAGCTTGTGCCTGTCCCTGAGTCCCAGTCCATCACGAGCAGCTGGTTT
CTAAGATGCTATTTCCCGTATAAAGCATGAGACCGTGACTTGCCAGCCCCACAGAGCCCC
GCCCTTGTCCATCACTGGCATCTGGACTCCAGCCTGGGTTGGGGCAAAGAGGGAAATGAG
ATCATGTCCTAACCCTGATCCTCTTGTCCCACAGATATCCAGAACCCTGACCCTGCCGTG
GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCT
GGACCTatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgag
ctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc
acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctgg
cccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccac
atgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacc
atcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgac
accctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag
aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcag
ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgac
aaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcac
atggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac
aagtaaTGATCATAATCAAGCCATATCACATCTGTAGAGGTTTACTTGCTTTAAAAAACC
TCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTT
TATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGC
ATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGT
CTGGATCTGCACCAGCTGAGAGACTCTAAATCCAGTGACAAGTCTGTCTGCCTATTCACC
GATTTTGATTCTCAAACAAATGTGTCACAAAGTAAGGATTCTGATGTGTATATCACAGAC
AAAACTGTGCTAGACATGAGGTCTATGGACTTCAAGAGCAACAGTGCTGTGGCCTGGAGC
AACAAATCTGACTTTGCATGTGCAAACGCCTTCAACAACAGCATTATTCCAGAAGACACC
TTCTTCCCCAGCCCAGGTAAGGGCAGCTTTGGTGCCTTCGCAGGCTGTTTCCTTGCTTCA
GGAATGGCCA
Quantity 50μg
Storage Condition Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Quality Specifications
Item Specifications
Purity (Agarose gel electrophoresis) Single band
Sequence accuracy (Sanger sequencing) 100% sequence alignment
Optical density (Spectrophotometry) 260 nm/230 nm ≥ 2.0; 260 nm/280 nm = 1.8~2.0
Bioburden No colony formation
Endotoxin (Qualitative TAL assay) ≤ 10 EU/mg
Protein Residue (Micro BCA Protein Assay Kit ) ≤50ug/mg

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