| Description |
ssDNA_RAB11A_GFP HDR donor template is high purity GenExactTMssDNA designed for inserting GFP into targeted RAB11A locus. Advantages of GenExactTM ssDNA donor template： ✔ Minimal cytotoxicity ✔ Precise knock-in, minimized off-target effect ✔ High purity and sequence verified ✔ Research to GMP grade Learn More>>> https://www.genscript.com/new-single-stranded-dna-synthesis-service.html This ssDNA HDR donor template works in combination with SafeEdit Rab11a sgRNA and Cas9 protein. [Reference] Roth. et al. Reprogramming human T cell function and specificity with non-viral genome targeting. Nature 2018, 559(7714): 405-409. |
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| Sequence |
GGTAGCTAGGAGTTCCAGGACTCAGTTTCCCCTTTGAGCCTCCTTTAGCGACTAAAGCTT GAAGCCCCACGCATCTCGACTCTCGCGCACACCGCCCTTGTTGGGCTCAGGGGCGGGGCG CCGCCCCCGGAAGTACTTCCCCTTAAAGGCTGGGGCCTGCCGGAAATGGCGCAGCGGCAG GGAGGGGCTCTTCACCCAGTCCGGCAGTTGAAGCTCGGCGCTCGGGTTACCCCTGCAGCG ACGCCCCCTGGTCCCACAGATACCACTGCTGCTCCCGCCCTTTCGCTCCTCGGCCGCGCA ATGGGCGGATCGGGTGGGACTAGTGGCAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTG CCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGCGCGGCGAG GGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAG CTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGC CGCTACCCCGACCACATGAAGCGCCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTAC GTCCAGGAGCGCACCATCAGCTTCAAGGACGACGGCACCTACAAGACCCGCGCCGAGGTG AAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAG GACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAACAGCCACAACGTCTATATC ACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACGTGGAG GACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCC GTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGTGCTGAGCAAAGACCCCAAC GAGAAGCGCGATCACATGGTCCTgCTGGAgTTCGTGACCGCCGCCGGGATCACTggaacc ggtGCTggaagtggtACaCGCGACGACGAGTACGACTACCTCTTTAAAGGTGAGGCCATG GGCTCTCGCACTCTACACAGTCCTCGTTCGGGGACCCGGGCCACTCCCGGTGGACCCTCG TGCCGGCCACCCCTGCACTGATATAGGCCTCCCTCAGCCCTTCCTTTTTGTGCGGTTCCG TCTCCTACCCAGCTCAGCCTCTTCTCCCCCGCTCAGACAGGGGTCCCCATCACATGCCGC TCTCTGAGCGACCTCTCCATAGGCCTTCGCTGGCCTCAGAGCCCCTCCCTGCGTGTCCTT CCCCTGGCGGACTGCCTTCTCCCACATCGT |
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| Quantity | 50μg | ||||||||||||||
| Storage Condition | Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles. | ||||||||||||||
| Quality Specifications |
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