Product Description |
The CytoSinct™ CD34 Nanobeads, human are
used for separating CD34+ cells from fresh or frozen peripheral blood mononuclear
cells (PBMCs), leukapheresis products or single cell suspension based on the
surface expression of human CD34. The surface of Nanobeads are labeled with
anti-human CD34 monoclonal antibody. To begin the isolation, Nanobeads are
added to the cells. The CD34 antibodies preconjugated to the Nanobeads can bind
the target cells expressing CD34 on cell surface. The cells/beads mixed
suspension is loaded onto a CytoSinct™
Column which is effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other compatible cell
isolation columns and magnets. The Nanobeads-labeled CD34+ cells are retained
within the column and enriched during the wash step when Isolation Buffer is
used to flush out the CD34- cells. After removing magnetic field, the target CD34+
cells can be easily eluted from the column.
Cat. No. | Name | Size | Capacity |
---|
L00967-1 | CytoSinct™ CD34 Nanobeads, human | 1 mL | for up to 1X109 total cells | |
Reactivity |
Human |
Format |
Bio-degradable
matrix coated nanoparticle conjugated with anti-CD34 antibodies supplied in
phosphate buffered-saline (PBS), containing Human Serum Albumin (HSA), pH
7.0-7.4. |
Application |
Positive selection or depletion of CD34+ cells
from leukapheresis, PBMC or cell cultures. Isolated CD34+ cells can be used for
culture and expansion, flow cytometry. |
Storage |
Store at 2-8 °C. Do not freeze. |
For research use only. Not intended for human and animal
therapeutic or diagnostic use.