ED₅₀<3 ng/ml, measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, corresponding to a specific activity of >3.3 x 10⁵ units/mg.

PDGF-BB, Human (P. pastoris-expressed)

Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.

产品编号	Z02529
规格	10 μg 50 μg 1 mg 大单询价
数量
价格	￥760


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产品简介

Species

Human

Protein Construction

PDGF-BB (Ser82-Thr190)
Accession # P01127

Purity

> 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC

Endotoxin Level

< 1 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 3.0 ng/ml, measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, corresponding to a specific activity of > 3.3 × 10⁵ units/mg.

Expression System

P. pastoris

Apparent Molecular Weight

~24.3 kDa, on SDS-PAGE under non-reducing conditions.

Formulation

Lyophilized after extensive dialysis against 10 mM acetic acid.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

图例

ED₅₀<3 ng/ml, measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, corresponding to a specific activity of >3.3 x 10⁵ units/mg.

技术背景

Target Background

Synonyms

PDGFBB; IBGC5; PDGF-2; PDGF2; SIS; SSV; c-sis; platelet derived growth factor subunit B

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.