| Species |
Human |
| Protein Construction |
Expressed with an N-terminal Met.
FGF-4 (Ala31-Leu206)
Accession # P08620 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 0.5 ng/ml, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2.0 × 106 units/mg. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~19.4 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against 50 mM HEPES, 750 mM NaCl, pH 7.5. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Lane 1: 2 μg of FGF-4, Human, reducing (R)
Lane 2: 2 μg of FGF-4, Human, non-reducing (NR)
> 95% as analyzed by SDS-PAGE
ED50 < 0.5 ng/ml, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2.0 × 106 units/mg.
| Target Background |
Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis. |
| Synonyms |
HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
