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目录产品 » 重组蛋白 » 细胞因子 » IFN-α 2a, Human
IFN-α 2a, Human

Biological Activity
IFN-alpha 2a, Human (Cat. No. Z03003) induced cytotoxicity of R&D TF-1 cells. The ED50 for this effect is less than 0.1ng/mL.

IFN-α 2a, Human

2 μg of IFN-α 2a, Human (Cat. No. Z03003) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

IFN-α 2a, Human

Interferon-Alpha 2a (IFN-Alpha 2a), Human produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2a shares 99.4% , 98.8% aa sequence identity with IFN-alpha 2b and 2c respectively. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.
Z03003
￥200

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产品简介
Species Human
Protein Construction
IFN-α 2a (Cys24-Glu188)
Accession # P01563
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.1 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 1.0 × 107 units/mg.
Expression System E. coli
Apparent Molecular Weight ~19.2 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

图例
  • IFN-α 2a, Human
    • IFN-α 2a, Human

    Biological Activity
    IFN-alpha 2a, Human (Cat. No. Z03003) induced cytotoxicity of R&D TF-1 cells. The ED50 for this effect is less than 0.1ng/mL.

  • IFN-α 2a, Human
    • IFN-α 2a, Human

    2 μg of IFN-α 2a, Human (Cat. No. Z03003) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.


技术背景
Target Background Interferon-Alpha 2a (IFN-Alpha 2a), Human produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2a shares 99.4% , 98.8% aa sequence identity with IFN-alpha 2b and 2c respectively. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.
Synonyms Leukocyte interferon; B cell interferon; Type I interferon; IFNA2; IFN-a 2a

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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