| Species |
Human |
| Protein Construction |
IL-4R (Gly24-His232)
Accession # P24394 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 70.0 ng/ml, measured in a neutralization assay using TF-1 cells in the presence of 0.5 ng/ml h-IL-4. |
| Expression System |
HEK 293 |
| Apparent Molecular Weight |
40~45 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
ED₅₀< 70 ng/ml, measured in a neutralization assay using TF-1 cells in the presence of IL-4,Human(Z02925).
| Target Background |
Interleukin-4 Receptor, also known as IL-4RA and CD124, is a transmembrane glycoprotein belonging to the class I receptor family. It is highly expressed by activated T-cells. IL-4RA couples with γ chain to form the type I receptor for IL-4. The extracellular domain of IL-4RA binds to IL-4 and antagonizes its activity. IL-4RA plays an important role in Th2 cell differentiation, Ig class switching and alternative macrophage activation. It has also been implicated in allergic inflammation, tumor progression and atherogenesis. |
| Synonyms |
IL4R; CD124; IL-4RA; IL4RA; Interleukin-4 receptor; interleukin 4 receptor |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
