| Species |
Human |
| Protein Construction |
| Poly-His |
TPO (Ser22-Gly353)
Accession # P40225 |
Poly-His |
| N-term |
|
C-term |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.The ED50 for this effect is typically 0.5-5.0 ng/ml. |
| Expression System |
Human Cells |
| Apparent Molecular Weight |
75~90 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5-5.0 ng/ml.
Lane 1: 3 μg of TPO, His, Human, non-reducing (NR)
Lane 2: 3 μg of TPO, His, Human, reducing (R)
> 95% as analyzed by SDS-PAGE
| Target Background |
Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. |
| Synonyms |
Thrombopoietin; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growthand development factor; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF; MKCSF; ML; MPLLG; THCYT1; TPO |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
