| Species |
Rat |
| Protein Construction |
IL-10 (Ser19-Asn178)
Accession # P29456 |
|
| Purity |
> 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC |
| Endotoxin Level |
< 0.1 EU/µg of protein by LAL method |
| Biological Activity |
The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1.0 ng/ml, corresponding to a specific activity of 1.0 × 106 IU/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
18.6 kDa |
| Formulation |
Lyophilized from a 0.2 µm filtered solution in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or -70°C. Upon reconstitution, the product should be stable for up to 1 week at 2-8°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine produced by a variety of cell lines including T-cells, macrophages and mast cells. IL-10 is classified as a class-2 cytokine, a set of cytokines including IL-19, IL-20, IL-22, IL-24, and IL-26. IL-10 can inhibit the synthesis of pro-inflammatory cytokines such as IFN-gamma, IL-2, IL-3, TNF and GM-CSF. It also stimulates Th2 responses, but suppresses the antigen-presentation capacity of antigen presenting cells. |
| Synonyms |
Cytokine synthesis inhibitory factor; CSIF; TGIF; IL10A |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
