| Species |
Bovine |
| Protein Construction |
Expressed with an N-terminal Met.
IGF-I (Gly50-Ala119)
Accession # P07455 |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 15.0 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 6.7 × 104 units/mg. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~7.7 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
ED50 < 5 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0× 105 units/mg.
| Target Background |
Insulin-like growth factor 1 (IGF-1), also called Somatomedin, is a hormone similar in molecular structure to insulin but has a much higher growth-promoting activity. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. It is able to stimulate glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. It may also play a role in synapse maturation. |
| Synonyms |
Somatomedin; IGF1; Insulin-like growth factor I; IGF-1 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
