目录产品 » FGF-basic, Salmon

The EC₅₀ as determined by a cell proliferation assay using mice Balb/c 3T3 cells is 0.053 ng/ml.

Lane 1: 2 µg of FGF-basic, Salmon, reducing (R)

Lane 2: 2 µg of FGF-basic, Salmon, non-reducing (NR)

The purity is greater than 95% as analyzed by SDS-PAGE

FGF-basic, Salmon

Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomyocytes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.

产品编号	Z03727
规格	10 μg 100 μg 1 mg 大单询价
数量
价格	￥560


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产品简介

Species

Salmon

Protein Construction

FGF-basic (Full length)
Accession # XP_035600424.1

Purity

≥ 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

EC₅₀ < 1.0 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the EC₅₀, the calculated specific activity is approximately > 1.0 × 10⁶ IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.

Expression System

E. coli

Theoretical Molecular Weight

17.1 kDa

Apparent Molecular Weight

~20 kDa, on SDS-PAGE under non-reducing conditions.

Formulation

Lyophilized from a 0.2 μm filtered solution in 7.8 mM Na₂HPO₄, 1.5 mM KH₂PO₄, 2.7 mM KCl, 500 mM NaCl.

Reconstitution

Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 °C.

图例

The EC₅₀ as determined by a cell proliferation assay using mice Balb/c 3T3 cells is 0.053 ng/ml.

Lane 1: 2 µg of FGF-basic, Salmon, reducing (R)

Lane 2: 2 µg of FGF-basic, Salmon, non-reducing (NR)

The purity is greater than 95% as analyzed by SDS-PAGE

技术背景

Target Background

Synonyms

FGF-2; BFGF; FGFB; FGF basic;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.